Microscopic Cell Tracking through Detection Identity and Lineage

Abstract

Microscopic cell tracking requires temporal identity and lineage evidence beyond accurate regions in individual frames. This article develops a structured measurement framework informed by 2026 tracking work and real published microscopy examples. Bright-field, phase-contrast, and fluorescence displays retain their source appearance. A constructed intensity field illustrates threshold dependence, known crossing trajectories isolate identity exchange, and analytic curves expose link-error accumulation and frame-interval effects. The proposed validation separates detections, links, complete graphs, and biological endpoints across independent recordings. It retains uncertainty, physical calibration, and review effort. No new segmentation benchmark, tracking leaderboard result, or biological experiment is claimed.